Aflatoxin M1 rapid test dipsticks (milk)
Catalog No. LSY-20038
1. Brief
This product is used for testing Aflatoxin M1 in milk sample (pure milk and fresh milk) and goat milk qualitatively. The whole test only need 13min, easy to operate with high sensitivity.
2. Detection limit
Milk: 0.5 ppb(ug/kg)
3. Contents
(1) 8 microwells and 8 dipsticks in one bottle 12 bottles / kit.
(2) Instruction 1 piece
4. Principle
The Aflatoxin M1 rapid test dipstick is based on competitive inhibition immuno-chromatographic principle. In the flow process, Aflatoxin M1 in the sample combined with Aflatoxin M1 specific colloidal gold-labeled monoclonal antibody, Inhibit the combination between antibody and Aflatoxin M1-BSA conjugate on Test line of NC membrane, lead to the color change of Test line. When the sample has no Aflatoxin M1 residue or concentration lower than detection limit, T line is darker than C line; when the concentration is equal to or higher than detection limit, T line is obviously lighter than C line or T line has no color or T line has same color with C line. No matter whether there is Aflatoxin M1 residue in sample, C line will appear, it means the test is valid.
5. Operation procedures
5.1 Read the instruction carefully before use. Return test dipsticks and sample into room temperature.
5.2 Take bottles needed from the kit package, take out required microwells and dipsticks, making proper marks. Please use these test dipsticks within 1h. Seal the cap of the bottles, avoid moisture.
5.3 Take 200ul of the test samples into the microwells, then repeatedly absorb for many times, mix the sample with the reagent in the microwells completely until no solid judged by eyes (this is a very important step).
5.4 Incubate for 5min at room temperature (20-25℃), insert the test dipsticks into the microwells with the "MAX" end fully dipped in to the mixture solution.
5.5 Incubate for 5-8min at room temperature again, read the result, it is invalid in other time.
6. Test Result Interpretation
|
Color depth comparison of T line and C line
|
Result judgment
|
Result analysis
|
|
T line >C line
|
Negative
|
Aflatoxins M1 residue is lower than detection limit in sample
|
|
T line =C line
|
Weak positive
|
Aflatoxins M1 residue is equal to detection limit in sample
|
|
T line <C line or T line is invisible
|
Positive
|
Aflatoxins M1 residue is higher than detection limit in sample
|
|
C line is invisible
|
Invalid
|
The test dipstick is invalid, test again
|
7. Precautions
1) The test card can be used only once at room temperature, do not use test card out of expiry date.
2) Please seal the bottle after taking out required test dipsticks. If can not use all 8 microwells at once, cup the rest and put it back to bottle and sealed.
3) Do not touch the white membrane surface in the middle of test card, avoid sunlight and fan blowing directly.
4) Milk samples should be fully liquid without any agglomeration and deposition.
5) Use the test dipsticks testing again for positive results.
6) Please contact the supplier for any questions.
7) The results is for reference only, for confirmation, please refer to the relevant national standard methods.
8.Specificity
Test 500ppb Sulfonamides, quinolones and tetracyclines and other drugs. All the results are negative.
9. Storage and expiry date
Storage: Store at 2-8 ℃ in dark, sealed, dry place, no frozen.
Expiry date: 12 months; date of production is on box.