Mycoplasma antibody rapid test kit (For pig, cattle and sheep)

Catalog No.: LSY-20062M

1. Introduction

The Mycoplasma antibody rapid test kit, based on colloidal gold method, uses rapid immunochromatography to detect antibodies to Mycoplasma in whole blood and serum of animals. After the sample is added to the addition hole, it moves along the chromatographic membrane with the colloidal gold labeled mycoplasma antigen. If anti-mycoplasma antibody is present in the sample, it binds to the antigen on the test line and shows Burgundy red. If no mycoplasma antibodies are present in the sample, no color response is produced.

2. Components

3. Test procedure

1) Collect whole blood, separate serum (centrifuge at 2000-3000 r/min for 5~15min or separate out naturally at 4℃ overnight). Or use whole blood with anticoagulant as test sample. Whole blood without anticoagulants may also be used, but must be tested immediately.

2) Open the package (Return to room temperature firstly if store in cold), take out test card, put it on clean desk flatly.

3) Use dropper to absorb the sample, adding 3 drop sample into “S” mark well slowly and vertically.

4) Wait for 10-20 minutes to read the results, the result is invalid after 20 minutes.

4. Results judgment

1) Positive: control line and test line are both seen wine red, the higher the antibody titer of Mycoplasma, the darker the detection line (T);

2) Negative: only control line is seen wine red;

3) Invalidation: control line isn’t seen wine red.

5. Notice

1) Do not use test card out of date and bad package;

2) Do not use pure water, running water and normal saline as negative control;

3) Store at room temperature (< 30℃) is OK. If store in cool, need return to room temperature firstly, then open package, avoid moisture. Use the test card as soon as possible after open the package.

4) Do not touch the white membrane surface in the middle of test card.

5) To ensure the test quality, take fresh, unpolluted sample. If there is a lot of blood lipids, it will affect the result, so after centrifuge, take serum to test, avoid fat. If the blood or serum is sticky or not enough, use normal saline to dilute at same dilution factor to dilute, then to test.

6) If the serum can not be tested immediately, store at 4℃ for short time (in 48hours), store at below -20℃ for long-tern storage.

7) Whole blood without anticoagulants should be tested immediately; if already exist part of cruor, separate serum to test. The whole blood with anticoagulants should be tested in 24hours, do not frozen. Freezing and thawing causes hemolysis of red blood cells rupture, seriously affect the test results.

8) When dropping sample, keep head of dropper and “S” well at 1cm to ensure adding sample accurately. If too close, can cause small droplet volume caused by drips judgment error or is not allowed to join the sample size, influence the test results.

9) Avoid bubble when adding sample, the sample quantity should be controlled at about 100ul (about 3 drops). Too much sample would not increase sensitivity. In particular, the whole blood of excessive have made erythrocyte sedimentation in "show hole" place, causing membrane chromatography is so deep into the red background, seriously affect the results.

10) The antibody level is only related to darkness of T line, no relation with C line. Only the C line is clean to see, it means the test is valid.

11) Treat the test card and sample at pollutant according to local regulations.

6. Packing: 40 tests per box

7. Storage and expiry date

Store at 2 - 30ºC and the shelf life is 24 months.

Shenzhen Lvshiyuan Biotechnology Co., Ltd

D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China

Tel. 86-755-28438788          Fax 86-755-28938800

Email:   info@lsybt.com

www.lsybt.com