Histamine rapid test kit (200ppm）
Catalog No. : GF107A\C4
1. Brief
This product is used for testing Histamine
residue in tissue sample. The total test time only need
15 ~20mins. High sensitivity, easy to operate.
2. Specifications: 10 tests/kit
3. Detection limit: 200mg/kg (ppm)
4. Application
Poultry and Livestock
tissue: tissue of pig, cow, sheep, chicken, duck, goose, etc.
Fish, shrimp, and
crab: tissue of freshwater fish, marine fish, shrimp, and crab.
5. Contents
|
Components
|
Quantity
|
|
1. Histamine test card
|
10
pieces
|
|
2. Instructions
|
1piece
|
|
3. Disposable plastic dropper
|
10
pieces
|
|
4. Desiccant
|
1
piece/bag
|
|
5. Sample extracting
buffer (50ml)
|
3
bottles
|
6. Principle
The histamine rapid test
card applies the principle of competitive suppression immunochromatography, and
the histamine in the sample binds to the specific histamine monoclonal antibody
labeled with colloidal gold during the flow process, which inhibits the binding
of the antibody and the histamine-BSA conjugate on the NC membrane testing
line, resulting in the color change of the testing line.
7.Required equipment and consumables
Instruments: electronic balance, centrifuge, micropipette, vortex shaker,
homogenizer.
Consumables: 50ml centrifuge tube, disposable tips.
8. Sample treatment
Weigh 1g ± 0.05g of tissue (homogenized by cutting), add 4ml of Sample
extracting buffer, shake for 1 minute, centrifuge at 4000rpm for 5 minutes,
take 100 µl of supernatant, add 8ml of Sample extracting buffer, mix well, and
take the mixed liquid of 100 µl+200 µl of Sample extracting buffer.
Ready for testing.
9.Operation
procedures
9.1 Read the
instructions carefully and return test card and sample into room temperature
before testing.
9.2 Take necessary test
card on desk flatly, make mark and use it in 1 hour.
9.3 Place the test card
flatly and use a disposable plastic dropper to vertically add 3 drops (or use a
micropipette to transfer 80μl) Sample into the sampling well.
9.4 Wait for 5 min, then
read the result.
9.5 Read result within
1min according to the picture, it’s invalid at other time.
10.Test Result Interpretation
|
Color depth comparison
of T line and C line
|
Result judgment
|
Result analysis
|
|
T line > C line or T
line = C line
|
Negative
|
No histamineresidue or lower than detection limit in sample
|
|
T line < C line or
T line is invisible
|
Positive
|
Histamineresidue is equal to or higher than detection limit in sample
|
|
C line is invisible
|
Invalid
|
The test card is
invalid, test again
|
11.Precautions
1) The test card can be
used only once at room temperature, do not use test card out of expiry date.
2) Every test
cassette and dropper is single use only, to avoid cross pollution.
3) Do not touch the
white membrane surface in the middle of test card, avoid sunlight and fan
blowing directly.
4) Tap water, distilled
water or deionized water cannot be taken as negative control sample.
5) Use this card to test
again when get positive result
6) Due to differences in
samples, some test line may appear light or gray in color, but as long as a red
band appears, it can be determined.
7) If you encounter any
problems during the testing, please contact our company.
12. Safety
performance and garbage disposal
The test card and its
packaging are non-toxic and harmless. After use, dispose of them as dry waste; Tissue
sample to be treated as wet waste.
13.Storage
and expiry date
Storage: Store at 4-30 ℃ in sealed,
dry, dark,no frozen.
Expiry
date:12 months; date of production is on box.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building,
National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng,
Shenzhen, 518120 China
Tel.
86-755-28438788
Fax
86-755-28938800
Email: info@lsybt.com
www.lsybt.com