Florfenicol rapid test strip (Tissue)
Catalog No. LSY-20079
1. Specifications:8 dipsticks/bottle, 12 bottles/box
2. Brief
This product is used for testing Florfenicol in tissue sample (including pork, chicken, fish, shrimp etc.) qualitatively. Easy to operate, high sensitivity.
3. Detection limit
Tissue: 0.1mg/kg (0.1ppb)
4.Contents
(1) 8 microwells and 8 dipsticks in one bottle 12 bottles / kit.
(2) Instruction 1 piece
(3) Sample Redissolving solution 1 bottle
5. Principle
The Florfenicol rapid test dipstick is based on competitive inhibition immuno-chromatographic principle. In the flow process, Florfenicol in the sample combined with Florfenicol specific colloidal gold-labeled monoclonal antibody, Inhibit the combination between antibody and Florfenicol-BSA conjugate on Test line of NC membrane, lead to the color change of Test line. When the sample has no Florfenicol residue or concentration lower than detection limit, T line is darker than C line or T line has same color with C line; when the concentration is equal to or higher than detection limit, T line is obviously lighter than C line or T line has no color. No matter whether there is Florfenicol residue in sample, C line will appear, it means the test is valid.
6. Materials needed but not provided.
Equipment: homogenizer, nitrogen-drying device (or hair dryer), vortex, oscillator (optinal), centrifuge, measuring pipets, balance (a sensibility reciprocal of 0.01 g).
Reagents: 50ml centrifuge tube, tips, ethyl acetate, n-Hexane.
7. Sample preparation
Tissue
1) Take 3±0.05g homogenized tissue sample into 50ml centrifuge tube, add 3ml deionized water, then add 7ml ethyl acetate, shake by hand or oscillator for 2min, centrifuge at 4000r/min for 5min;
2) Take 4ml supernatant into another centrifuge tube, blow to dry at 56℃by Nitrogen or air (or use hair dryer).
3) Add 0.5ml n-Hexane, shake for 30s (or repeatedly blow and beat until Re-dissolved), dissolve substance on tube wall and bottom completely, then add 0.45ml Sample Redissolving solution, shake for 10s, be static or centrifuge at 4000r/min for 5min until solution stratification, discard up-layer n-Hexane phase, absorb 200ul down-layer solution for test.
8.Operation procedures
8.1 Read the instruction carefully before use. Return test dipsticks and sample into room temperature.
8.2 Take bottles needed from the kit package, take out required microwells and dipsticks, making proper marks. Please use these test dipsticks within 1h. Seal the cap of the bottles, avoid moisture.
8.3 Take 200ul of the test samples into the micro-wells, then repeatedly absorb for many times, mix the sample with the reagent in the micro-wells completely (this is a very important step).
8.4 Incubate for 5min at room temperature (20-25℃), insert the test dipsticks into the micro-wells with the "MAX" end fully dipped in to the mixture solution.
8.5 Insert the test dipsticks into micro-wells for 3-5min then read the result, it is invalid in other time.
9. Test Result Interpretation
9.1 Negative: T line is darker than C line, or T line has same color with C line. It means there is no Florfenicol residue in sample or the residue is lower than detection limit.
9.2 Positive: T line is obviously lighter than C line or T line is invisible. It means the Florfenicol residue is equal to or higher than detection limit.
9.3 Invalidation: C line isn’t seen wine red. It means the test card is out of efficacy, out of date or improper operation. Please run the test again using another package. If the invalid tests keep happening, please contact the supplier.
10.Precautions
1) The test dipsticks can be used only once at room temperature, do not use test dipsticks out of expiry date.
2) Do not touch the white membrane surface in the middle of test dipsticks, avoid sunlight and fan blowing directly.
3) Use the test dipsticks testing again for positive results.
4) Please contact the supplier for any questions.
11.Specificity
Test 500ppb Sulfonamides, quinolones and tetracyclines and other drugs. All the results are negative.
12.Storage and expiry date
Storage: Store at 2-8 ℃ in dark, sealed, dry place, no frozen.
Expiry date: 12 months; date of production is on box.