Florfenicol rapid test strip (Egg)
Catalog No. LSY-20079
1. Specifications:8 dipsticks/bottle, 12 bottles/box
2. Brief
This product is used for testing Florfenicol in egg, duck egg sample qualitatively. The whole test only need 10min, Easy to operate, high sensitivity.
3. Detection limit
Tissue: 0.5ng/ml (0.5ppb)
4.Contents
(1) 8 microwells and 8 dipsticks in one bottle 12 bottles / kit.
(2) Instruction 1 piece
5. Principle
The Florfenicol rapid test dipstick is based on competitive inhibition immuno-chromatographic principle. In the flow process, Florfenicol in the sample combined with Florfenicol specific colloidal gold-labeled monoclonal antibody, Inhibit the combination between antibody and Florfenicol-BSA conjugate on Test line of NC membrane, lead to the color change of Test line. When the sample has no Florfenicol residue or concentration lower than detection limit, T line is darker than C line or T line has same color with C line; when the concentration is equal to or higher than detection limit, T line is obviously lighter than C line or T line has no color. No matter whether there is Florfenicol residue in sample, C line will appear, it means the test is valid.
6. Sample preparation
Egg
Take 1g fresh homogeneous egg sample, add 3ml pure water, shake by hand or vortex strongly for 3min, ready for testing.
7.Operation procedures
7.1 Read the instruction carefully before use. Return test dipsticks and sample into room temperature.
7.2 Take bottles needed from the kit package, take out required microwells and dipsticks, making proper marks. Please use these test dipsticks within 1h. Seal the cap of the bottles, avoid moisture.
7.3 Take 200ul of the test samples into the micro-wells, then repeatedly pump and suck for five times, mix the sample with the reagent in the micro-wells completely until no solid judged by eyes (this is a very important step).
7.4 Incubate for 5min at room temperature (20-25℃), insert the test dipsticks into the micro-wells with the "MAX" end fully dipped in to the mixture solution.
7.5 Insert the test dipsticks into micro-wells for 5min then read the result, it is invalid in other time.
8. Test Result Interpretation
8.1 Negative: T line is darker than C line, or T line has same color with C line. It means there is no Florfenicol residue in sample or the residue is lower than detection limit.
8.2 Positive: T line is obviously lighter than C line or T line is invisible. It means the Florfenicol residue is equal to or higher than detection limit.
8.3 Invalidation: C line isn’t seen wine red. It means the test card is out of efficacy, out of date or improper operation. Please run the test again using another package. If the invalid tests keep happening, please contact the supplier.
9.Precautions
1) The test dipsticks can be used only once at room temperature, do not use test dipsticks out of expiry date.
2) Please seal the bottle after taking out required test strips. If can not use all 8 micro-wells at once, cup the rest and put it back to bottle and sealed.
3) Do not touch the white membrane surface in the middle of test dipsticks, avoid sunlight and fan blowing directly.
4) Egg samples must be homogeneous and free of agglomeration, acidification and precipitation.
5) Use the test dipsticks testing again for positive results.
6) Please contact the supplier for any questions.
7) The result is for reference only, for confirmation, please refer to the relevant national standard method.
10. Specificity
Test 500ppb Sulfonamides, quinolones and tetracyclines and other drugs. All the results are negative.
11.Storage and expiry date
Storage: Store at 2-8 ℃ in dark, sealed, dry place, no frozen.
Expiry date: 12 months; date of production is on box.