Ehrlichia Canis (E.canis Ab) Antibody Rapid Test Kit
Catalog No.: CK04
1. Introduction
The Ehrlichia Canis (E.canis Ab) Antibody Rapid Test Kit is an immuno-chromatographic test, using colloidal gold immunoassay method, to detect the antibody against Ehrlichia Canis in serum, plasma or whole blood of dogs qualitatively. After adding sample, the sample move along with the colloidal gold labeling E.canis antigen, if there is E.canis antibody in the sample, it combines with antigen on T line and show wine-red color; if there is no E.canis antibody, then no color reaction.
2. Contents
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1
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E.canis Ab test
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40 pieces
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2
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Dropper
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40 pieces
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3
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Dilution Buffer
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4 bottles
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4
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Manual
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1 piece
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3. Test procedure
1) Collect whole blood, separate serum or plasma (centrifuge at 2000-3000 r/min for 5~15min or separate out naturally at 4℃overnight), or take whole blood with anticoagulants as sample. Take whole blood without anticoagulants as sample is also OK, but must test it immediately.
2) Open the package, take out test card, put it on clean, flat desk.
3) Use the dropper to add 10ul sample (sample quantity at the black line position of dropper) into “S” well on test card.
4) Then add 3 drops of Dilution Buffer into “S” well on test card, there will be wine-red color liquid pass by the observation window, if no liquid movement, press gently on the zone between “S”well and “observation window” to help liquid release.
5) Put it at room temperature for 5-10min, then read the result, the result is invalid after 10min.
4.Results judgment
1) Positive: control line and test line are both seen red color;
2) Negative: only control line is seen wine red;
3) Invalidation: control line isn’t seen wine red.
5. Note
1) Do not use product with broken Aluminum foil bag package and out of date.
2) Store at room temperature (< 30℃) is OK. If store in cool, need return to room temperature firstly, then open package, avoid moisture. Use the test card as soon as possible after open the package.
3) Do not touch the white membrane surface in the middle of test card.
4) To ensure the test quality, take fresh, unpolluted sample. If there is a lot of blood lipids, it will affect the result, so after centrifuge, take serum to test, avoid fat.
5) If the serum can not be tested immediately, store at 4℃for short time (in 48hours), store at below -20℃for long-tern storage.
6) Whole blood without anticoagulants should be tested immediately; if already exist part of cruor, separate serum to test. The whole blood with anticoagulants should be tested in 24hours, do not frozen. Freezing and thawing causes hemolysis of red blood cells rupture, seriously affect the test results.
7) Treat the used test card and sample as pollutant according to local regulations.
6. Storage and expiry date
Specifications: 40T/kit
Storage: Store at 2-30 ℃.
Expiry date: 24 months; date of production is on box.