Chloramphenicol(CAP) rapid test dipsticks (Honey)

Catalog No. LSY-20004

1. Brief

This product is used for testing Chloramphenicol in honey sample qualitatively. The whole test need 15`30min. Easy to operate, high sensitivity.

2. Detection limit

Honey:  0.5ng/ml (0.5ppb)

3. Contents

4. Principle

The Chloramphenicol rapid test dipstick is based on competitive inhibition immuno-chromatographic principle. In the flow process, Chloramphenicol in the sample combined with Chloramphenicol specific colloidal gold-labeled monoclonal antibody, Inhibit the combination between antibody and Chloramphenicol-BSA conjugate on Test line of NC membrane, lead to the color change of Test line. When the sample has no Chloramphenicol residue or concentration lower than detection limit, T line is darker than C line or T line has same color with C line; when the concentration is equal to or higher than detection limit, T line is obviously lighter than C line or T line has no color. No matter whether there is Chloramphenicol residue in sample, C line will appear, it means the test is valid.

5. Sample preparation

1) Sampleextract A: Dilute 10X concentrated sample buffer with deionized water at 1:9 to get sample extract A. (1 part 10X concentrated sample buffer + 9 part deionized water)

2) Honey:weigh 2±0.05g honey sample, add 3ml Sample extract A, shake for 3min; add 300ul Sample extract B, shake for 1min; add 2ml Sample extract C, shake for 1min; ready to test.

6.Operation procedures

6.1 Read the instruction carefully before use. Return test dipsticks and sample into room temperature.

6.2 Take bottles needed from the kit package, take out required microwells and dipsticks, making proper marks. Please use these test dipsticks within 1h. Seal the cap of the bottles, avoid moisture.

6.3 Take 200ul of the test samples into the microwells, then repeatedly absorb for 5 times, mix the sample with the reagent in the microwells completely, until no solid observed by eyes (this is a very important step).

6.4 Incubate for 5min at room temperature (20-25℃), insert the test dipsticks into the microwells with the "MAX" end fully dipped in to the mixture solution.

6.5 Insert the test dipsticks in microwells for 8-10min, read the result, it is invalid in other time.

7. Test Result Interpretation

7.1 Negative: T line is darker than C line, or T line has same color with C line. It means there is no Chloramphenicol residue in sample or the residue is lower than detection limit.

7.2 Positive: T line is obviously lighter than C line or T line is invisible. It means the Chloramphenicol residue is equal to or higher than detection limit.

7.3 Invalidation: C line isn’t seen wine red. It means the test card is out of efficacy, out of date or improper operation. Please run the test again using another package. If the invalid tests keep happening, please contact the supplier.    

8.Precautions

1) The test card can be used only once at room temperature, do not use test card out of expiry date.

2) The tips is disposable, do not repeat use to avoid cross pollution.

3) Do not touch the white membrane surface in the middle of test card, avoid sunlight and fan blowing directly.

4) Tap water, distilled water or deionized water can not be Negative control. .

5) Please contact the supplier for any questions.

9.Storage and expiry date

Storage:  Store at 2-8 ℃ in dark, sealed, dry place, no frozen.

Expiry date: 12 months; date of production is on box.