Oxytetracyline rapid test dipstick (Honey)

Catalog No. LSY-20083

1. Brief

This product is used for testing Oxytetracyline residue in honey sample qualitatively. The total test time need 15-30 min. Easy to operate, high sensitivity.

2. Detection limit:30-40ppb

3. Specifications: 8 dipsticks/bottle, 12 bottles/box

4. Principle

The Oxytetracyline rapid test dipstick is based on competitive inhibition immuno-chromatographic principle. In the flow process, Oxytetracyline in the sample combined with Oxytetracyline specific colloidal gold-labeled monoclonal antibody, Inhibit the combination between antibody and Oxytetracyline -BSA conjugate on Test line of NC membrane, lead to the color change of Test line.

When the sample has no Oxytetracyline residue or concentration lower than detection limit, T line is darker than or has same color with C line; when the concentration is equal to or higher than detection limit, T line has no color or T line is lighter than C line. No matter whether there is Oxytetracyline residue in sample, C line will appear, it means the test is valid.  

5.Contents

6. Sample preparation

Dilute 10XSampleextraction A with deionized water at 10 times before use. (1 part 10X Sampleextraction A + 9 parts deionized water)

Sample preparation

Weigh 2±0.05ghoney, add 3ml diluted Sample extraction A, shake for 3min; then add300ulSample extraction B, shake for 1min; add 2ml Sample extraction C,shake for 1min, ready to test.

7.Operation procedures

7.1 Read the instruction carefully before use. Return test dipsticks and sample into room temperature.

7.2 Take bottles needed from the kit package, take out required micro-wells and dipsticks, making proper marks. Please use these test dipsticks within 1h. Seal the cap of the bottles, avoid moisture. 

7.3 Take 200ul of the test samples into the micro-wells, then repeatedly pump and suck for 5 times, mix the sample with the reagent in the micro-wells completely until no solid judge by eyes (this is a very important step).

7.4 Incubate for 5min at room temperature (20-25℃), then insert the test dipsticks into the micro-wells with the "MAX" end fully dipped in to the mixture solution.

7.5 Insert the dipstick into the microwell for 8-10min, read the result, it is invalid in other time.

8. Test Result Interpretation

8.1 Negative: C line appears, T line is darker than C line, or T line has same color with C line. It means there is no Oxytetracyline residue in sample or the residue is lower than detection limit.

8.2 Positive: C line appears, T line is obviously lighter than C line or T line is invisible. It means the Oxytetracyline residue is equal to or higher than detection limit.

8.3 Invalidation: C line isn’t seen wine red. It means the test card is out of efficacy, out of date or improper operation. Please run the test again using another package. If the invalid tests keep happening, please contact the supplier.

9.Specificity

This product has no cross reaction with Chloramphenicol, Quinolones, Streptomycin etc.

10.Precautions

1) The test strips can be used only once at room temperature, do not use strips out of expiry date.

2) Every tips is single use only, to avoid cross pollution.

3) Do not touch the white membrane surface in the middle of test strip, avoid sunlight and fan blowing directly.

4) Tap water, distilled water and deionized water can not treat as negative control.

5) Please contact the supplier for any questions.

11.Storage and expiry date

Storage:  Store at 2-8 ℃ in dark, sealed, dry place, no frozen.

Expiry date: 12 months; date of production is on box.