Metronidazole rapid test strips (Honey)

Catalog No. LSY-20120

1. Specifications:8 dipsticks/bottle, 12 bottles/box

2. Brief

This product is used for testingMetronidazole in honey sample qualitatively.The whole test only need 5~10 min, easy to operate with high sensitivity.

3. Detection limit: 10ng/g (10ppb)

4. Contents

5. Principle

TheMetronidazole rapid test strip isbased oncompetitive inhibition immuno-chromatographic principle. In the flow process, Metronidazole in the sample combined withMetronidazole specific colloidal gold-labeled monoclonal antibody, Inhibit the combination between antibody andMetronidazole-BSA conjugate on Test line of NC membrane, lead to the color change of Test line. When the sample has noMetronidazole residue or concentration lower than detection limit, T line is darker than C line or T line has same color with C line; when the concentration is equal to or higher than detection limit, T line is obviously lighter than C line or T line has no color. No matter whether there isMetronidazole residue in sample, C line will appear, it means the test is valid.

6.Sample preparation

Honey

Take 1±0.05g honey sample into 50ml centrifuge tube, add 4ml pure water, shake for 3min, until the honey sample to be dissolved, ready for testing.

7.Operation procedures

7.1 Read the instruction carefully before use. Return teststrips and sample into room temperature.

7.2Take bottles needed from the kit package, take out requiredmicrowells andstrips, making proper marks. Please use these teststrips within 1h. Seal the cap of the bottles, avoid moisture.

7.3Take200ul of the test samples into themicro-wells, then repeatedly absorb formany times, mix the sample with the reagent in themicro-wells completely until no solid judge by eyes(this is a very important step).

7.4Incubate for 5min at room temperature (20-25℃),theninsert the teststrips into themicro-wells with the "MAX" end fully dipped in to the mixture solution.

7.5Insert the test strips into micro-wells for 5minthen read the result, it is invalid in other time.

8. Test Result Interpretation

8.1Negative:T line is darker than C line, or T line has same color with C line. It means there is noMetronidazole residue in sample or the residue is lower than detection limit.

8.2Positive: T line is obviously lighter than C line orT line is invisible.It means theMetronidazole residue is equal to or higher than detection limit.

8.3 Invalidation:C line isn’t seen wine red. Itmeans the test card is out of efficacy, out of date or improper operation. Please run the test again using another package. If the invalid tests keep happening, please contact the supplier.    

9.Precautions

1) The testdipstickscan be used only once at room temperature, do not use testdipsticks out of expiry date.

2)Please seal the bottle after taking out required test dipsticks. If can not use all 8 micro-wells at once, cup the rest and put it back to bottle and sealed.

3) Do not touch the white membrane surface in the middle of testdipsticks, avoid sunlight and fan blowing directly.

4)Tap water, distilled or deionized water cannot be used as negative controls

5) Use the test dipsticks testing again for positive results.

6) Please contact the supplier for any questions.

7) The result is for reference only, for confirmation, please refer to the relevant national standard method.

10.Specificity

Test 500ppbSulfonamides and tetracyclines and other drugs. All the results are negative.

11. Storage and expiry date

Storage:  Store at2-8 ℃ in dark, sealed, dry place, no frozen.

Expiry date: 12 months; date of production is on box.