Aflatoxin B1 fluorescence quantitative rapid test card

Catalog No.: IF001A01

[Brief]

This product is used for rapid quantitative detection of aflatoxin B1 in grains and their products, vegetable oils, feed raw materials, and some finished feed products. It is suitable for on-site rapid detection by various enterprises, grain depots, testing institutions, and government regulatory departments.

[Principle]

This product is based on the principle of competition and combined with fluorescence chromatography technology. The aflatoxin B1 (AFB1) in the sample competes with the AFB1 Ag coated on the NC membrane testing line for fluorescent microsphere labeled AFB1 monoclonal antibody. As the AFB1 content in the test sample increases, the amount of fluorescent microsphere antibody complex bound to the testing line decreases, and the fluorescence intensity of the testing line weakens accordingly. After the reaction is completed, the content of AFB1 in the sample can be detected using a matching handheld fluorescence immunoassay analyzer.

[Application]

Grains and their products, vegetable oils, feed raw materials, and some finished feed products.

[Specification]

20 tests/box, 50 tests/box

[Product performance]

Detection range: 0～100μg/kg(ppb)

Detection limit: 2μg/kg(ppb)

Recovery rate: 80%～120%

Precision: intra batch CV≤20%, inter batch CV≤20%

[Components]

[Required equipment, consumables, and reagents] (Not provided)

1. Equipment and consumables: electronic balance, oscillator, centrifuge, pipette, and tips (20-200μL,100-1000μL, 1-5mL), centrifuge tube (50mL, 1.5mL), disposable gloves and mask.

2. Reagent: Anhydrous ethanol (AR).

[Reagent preparation]

Sample extraction solution: Take 500mL of anhydrous ethanol and add 500mL of pure water, mix well for usage.

[Experimental Steps]

1. Preparation before testing

a. Return the test card and sample diluent to room temperature (25℃) for at least 30 minutes.

b. Turn on the side power switch of the handheld fluorescence immunoassay analyzer, enter the password 123456 and click the arrow in the bottom right corner of the keyboard to complete the input. Click login to enter the main interface of the instrument and preheat for 10 minutes.

c. Turn on the constant temperature timer, set the temperature to 37℃, and incubate for 8 minutes.

2. Sample preparation

Multiple point sampling is required for the test sample, with a total sampling amount of 300-500g. After thorough mixing, the particles can be homogenized to over 90% and pass through a 20 mesh sieve. Extract the screened samples for testing.

a. Conventional samples

Weigh 5.0±0.05g of homogeneous sieved sample into a 50mL centrifuge tube, add 25mL of sample extraction solution, shake for 3 minutes, and centrifuge at 4000rpm for 5 minutes. Take 100μL supernatant, add 700μL sample diluent, shake and mix well for 10 seconds, ready for testing.

b. Bran sample

Weigh 2.5± 0.05g of homogeneous sieved sample into a 50mL centrifuge tube, add 25mL of sample extraction solution, shake for 3 minutes, and centrifuge at 4000rpm for 5 minutes. Take 100μL supernatant, add 700μL sample diluent, shake and mix well for 10 seconds, ready for testing.

c. Vegetable oil samples

Weigh 5.0±0.05g of the mixed sample into a 50mL centrifuge tube, add 25mL of sample extraction solution, shake for 3 minutes, and centrifuge at 4000rpm for 5 minutes. Take 100μL down-layer clear liquid, add 700μL sample diluent, shake and mix well for 10 seconds, ready for testing.

3. Sample testing

a. Take out the warmed test card from the aluminum foil bag, place it on a horizontal table, and use a pipette to aspirate 70μL of the diluted sample, add into the sample hole, insert the test card into the constant temperature timer socket, and incubate for 8 minutes;

b. Click on the "Test" module of the handheld fluorescence immunoassay analyzer, insert the ID card into the ID card socket, click "Select Project", click "Read Project Card", click "Confirm", and the name of the read project will appear in the box below "Select Project". After selecting the required testing project and barcode, it will automatically return to the testing page.

c. After incubation, select the corresponding sample type curve according to the instructions, insert the test card with the sample hole end facing inward and left into the instrument, and click "Test" on the screen for detection.

Sample type corresponding to sample kind (standard curve)

Sample kind (standard curve)	Applicable sample types
Sample kind 1	Samples and processed products of grains such as wheat, corn, rice, peanuts, and vegetable oils
Sample kind 2	Corn processing by-products
Sample kind 3	DDGS、some finished feed and some meal category
Sample kind 4	bran

4. Result acquisition

The test results will be displayed on the screen of the handheld fluorescence immunoassay analyzer. You can click to print to obtain a paper printed report, or export an electronic version of the data report through a data cable.

Note: When the concentration of the analyte in the sample exceeds the range of the curve, the sample extraction solution can be used to dilute the extracted supernatant (or down-layer clear liquid) by 2-5 times, then take 100μL diluted extraction solution, add 700μL sample diluent, mix thoroughly and add the sample for testing. The final result is obtained by multiplying dilution factor.

[Precautions]

1. Please use the test card within its validity period, and each card can only be used once;

2. Please use the test card immediately when opening the aluminum foil bag packaging;

3. Different batches of test cards, sample diluents, and ID cards should not be mixed;

4. Do not touch the white film strip at the detection window. If touched, please discard this card to avoid affecting the detection results;

5. After incubating with the sample for 8 minutes, please test immediately and try to complete the test within 10 minutes after adding the sample;

6. During the testing process, reagents, instruments, etc. should be avoided from direct sunlight, fans, and air conditioning;

7. This product is only used for screening. If a positive result is found, it is recommended to retest by HPLC or LC-MS;

8. If you encounter any problems during testing, please contact the supplier.

9. The disposal of experimental waste shall be carried out in accordance with national or local laws and regulations.

[Storage and shelf life]

1. This product should be stored in a dry and dark place at 2-8 ℃, and should not be frozen.

2. The shelf life is 12 months. The batch number and production date can be found in the outer packaging.

Shenzhen Lvshiyuan Biotechnology Co., Ltd

D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China

Tel. 86-755-28438788      Fax 86-755-28938800

Email: info@lsybt.com      Website: www.lsybt.com