一、产品简介

本试剂盒采用间接竞争ELISA方法，在微孔条上预包被偶联抗原，样本中的残留物磺胺类药物将和微孔条上预包被的偶联抗原竞争抗磺胺类药物抗体，加入酶标记物后，用TMB底物显色，样本吸光值与其所含残留物磺胺类药物的含量成负相关，与标准曲线比较即可得出相应残留物磺胺类药物的含量。

二、适用范围

可定性定量检测相应残留物中物磺胺类药物的含量。（部分样本可定制，具体咨询客服人员）

三、试剂盒特性

试剂盒灵敏度：1ppb

孵育温度：25℃

孵育时间：30min~15min

样本检测下限

组织（高检测限方法）··············1ppb

组织（低检测限方法）··············5ppb

蜂蜜、鸡蛋···················1ppb

血清、尿液···················4ppb

牛奶······················20ppb

交叉反应率

磺胺甲基嘧啶（SM1）··············111.5%

磺胺嘧啶（SD或SDZ）·············130.2%

磺胺间（六）甲氧嘧啶（SMM）·········181.8%磺胺对甲氧嘧啶（SMD）···················194.8%

磺胺二甲基嘧啶（SM2）·············89.3%

磺胺二甲异嘧啶（SM2′）············70.6%

磺胺间二甲氧嘧啶（SDM）············140.8%

磺胺邻二甲氧嘧啶（SDM′）···········132.1%

磺胺甲噁唑（SMZ）···············86.6%

磺胺多辛（SDM2)················144.7%

酞酰磺噻唑（PST）···············73.9%

磺胺甲噻二唑（SMT）··············69.4%

磺胺异噁唑（SIZ）················76.5%

磺胺噻唑（ST）·················103.3%

磺胺喹噁林(SQX)················101.5%

磺胺甲氧哒嗪(SMP)···············108.6%

磺胺氯吡嗪(Esb3)················72.1%

磺胺氯哒嗪(SCPA)···············59.6%

磺胺苯酰(SML)················104%

由反应交叉率可以得出：该试剂盒可用于磺胺多种残留物的检测，完全满足国家磺胺类多残留种类检测的要求。

样本回收率

组织、尿样、牛奶···············95%±25%

蜂蜜、鸡蛋、血清···············85%±25%

四、样品前处理步骤及检测分析步骤

详见产品说明书。

五、储藏条件和保质期

1、储藏条件：试剂盒于2～8℃保存，不要冷冻。

2、保质期：该产品有效期为1年。