一、产品简介
本试剂盒采用间接竞争ELISA方法,在微孔条上预包被偶联抗原,样本中的残留物克伦特罗将和微孔条上预包被的偶联抗原竞争抗克伦特罗抗体,加入酶标记物后,用TMB底物显色,样本吸光值与其所含残留物克伦特罗的含量成负相关,与标准曲线比较即可得出相应残留物克伦特罗的含量。
二、适用范围
可定性定量检测相应残留物中克伦特罗的含量。(部分样本可定制,具体咨询客服人员)
三、试剂盒特性
u 试剂盒灵敏度: 0.1ppb
u 孵育温度: 25℃
u 孵育时间: 30min~15min
u 样本检测下限
尿 样 ······························ 0.1ppb
组 织(处理法一) ····························· 0.4ppb
组 织(处理法二) ····························· 0.1ppb
饲 料 ································ 1ppb
u 交叉反应率
克伦特罗(Clenbuterol) ························· 100%
特普他林(Terbutalin) ······················· <1%
马布特罗(Mabuterol) ······················· <1%
溴布特罗(Brombuterol) ······················· <1%
沙丁胺醇(Salbutamol) ·························· <1%
莱克多巴胺(Ractopamine)·························<1%
u 样本回收率
尿 样 ································ 95%±22%
组 织 ································ 75%±20%
饲 料 ································ 80%±20%
四、样品前处理步骤及检测分析步骤
详见产品说明书。
四、储藏条件和保质期
1、 储藏条件:试剂盒于2-8℃保存,不要冷冻。
保 质 期:该产品有效期为1年,生产日期见包装盒。