Chinese  
   
呕吐毒素酶免检测试剂盒(检饲料/粮食,灵敏度3ppb,一步法)

                     14538747479742613.png

呕吐毒素酶免检测试剂盒(检饲料/粮食,灵敏度3ppb,一步法)

一、原理

本试剂盒采用间接竞争ELISA方法,在微孔条上预包被偶联抗原,样本中的残留物呕吐毒素将和微孔条上预包被的偶联抗原竞争呕吐毒素抗体,加入酶标记物后,用TMB底物显色,样本吸光值与其所含残留物呕吐毒素的含量成负相关,与标准曲线比较即可得出相应残留物呕吐毒素的含量。


二、试剂盒特性

u  试剂盒灵敏度:   3ppb

u  孵育温度:       25

u  孵育时间:       30min15min

u  样本检测下限

饲    料          ······························································································   300 ppb

花生、大米、玉米   ················································································    150 ppb

u  交叉反应率

呕 吐 毒 素  ································································································     100%

u  样本回收率

花生、大米、玉米、饲料  ··········································································   95±25%


三、试剂盒组成

1

微量测试孔

每条8孔,一板12

2

标准液×6

1ml/瓶)

0ppb

3ppb

9ppb

27ppb

81ppb

243ppb

3

酶标记物

7ml

红色帽

4

抗体工作液

7ml

蓝色帽

5

底物A

7ml

白色帽

6

底物B

7ml

黑色帽

7

终止液

7ml

黄色帽

8

20X浓缩洗涤液

15ml

白色帽

9

5X浓缩复溶液

100ml

透明帽


四、储藏条件和保质期

1、 储藏条件:试剂盒于28℃保存,不要冷冻。

2、 期:该产品有效期为1年,生产日期见包装盒。


 

农业部生物毒素检测重点实验室

联合研制