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地塞米松ELISA快速检测试剂盒(检组织/饲料/乳制品/蜂蜜/尿样,灵敏度0.1ppb,一步法)

                              

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 地塞米松ELISA快速检测试剂盒

一、原理

    地塞米松是肾上腺皮质激素类药物之一,有抗炎、抗休克、抗过敏、抗毒素等作用。养殖环节用药管理不严或者不科学,超量使用或没有严格执行休药期将造成地塞米松残留超标。农业部第235号公告《动物性食品中兽药最高残留限量》规定,地塞米松在牛的肌肉中的残留限量为0.75μg/kg。人们长期食用含低剂量地塞米松的动物性食品,可能会在人体内蓄积,干扰人体的激素分泌体系和其它正常代谢。

本试剂盒采用间接竞争ELISA方法,在微孔条上预包被偶联抗原,样本中的残留物地塞米松药物将和微孔条上预包被的偶联抗原竞争抗地塞米松药物抗体,加入酶标记物后,用TMB底物显色,样本吸光值与其所含残留物地塞米松药物的含量成负相关,与标准曲线比较即可得出相应残留物地塞米松药物的含量。

二、试剂盒特性

u  试剂盒灵敏度:       0.1ppb

u  孵 育 温 度 :       25

u  孵 育 时 间 :       30min15min

u  样本检测下限

      ·····································  0.1 ppb

      ·····································  0.2 ppb

      ·····································  0.3 ppb

      ·····································  0.2 ppb

     ·····································  0.2 ppb

尿      ·····································  0.2 ppb

u  交叉反应率

地塞米松  ········································  100%

u  样本回收率

  饲料      ·························································   90% ±25%

     粉              ························································   85% ±25%

尿 样、牛 奶、蜂 蜜  ······················································  95% ±25%

三、试剂盒组成

1

微量测试孔

每条8孔,一板12

2

标准液×6

1ml/瓶)

0ppb

0.1ppb

0.3ppb

0.9ppb

2.7ppb

8.1ppb

3

酶标记物

7ml

红色帽

4

抗体工作液

7ml

蓝色帽

5

底物A

7ml

白色帽

6

底物B

7ml

黑色帽

7

终止液

7ml

黄色帽

8

5X浓缩复溶液

55ml×2

透明帽

9

20X浓缩洗涤液

15ml

白色帽

四、样本前处理步骤及检测分析步骤

      详见产品说明书。

五、储藏条件和保质期

1、 储藏条件:试剂盒于2-8保存,不要冷冻。

2、 期:该产品有效期为1年,生产日期见包装盒。