Chinese  
   
四环素类ELISA快速检测试剂盒(检鸡肉/猪肉/蜂蜜,灵敏度0.05ppb,一步法)

14538747479742613.png                   

四环素ELISA快速检测试剂盒

一、原理

本试剂盒采用间接竞争ELISA方法,在微孔条上预包被偶联抗原,样本中的残留物四环素将和微孔条上预包被的偶联抗原竞争抗四环素抗体,加入酶标记物后,用TMB底物显色,样本吸光值与其所含残留物四环素的含量成负相关,与标准曲线比较即可得出相应残留物四环素的含量。

二、试剂盒特性

u  试剂盒灵敏度:  0.05ppb

u  孵育温度:      25

u  孵育时间:      30min15min

u  样本检测下限

鸡肉样本:

四环素 ···············································  0.4ppb

二甲胺四环素 ··································  0.4ppb

吡四环素 ··········································  0.4ppb

金霉素   ············································  0.4ppb

脱甲基金霉素 ···································  1.2ppb

土霉素 ··············································  0.8ppb

强力霉素 ··········································   1ppb

猪肉、蜂蜜样本:

四环素 ··········································  0.5ppb

二甲胺四环素 ·································  0.5ppb

吡四环素 ······································   0.5ppb

金霉素 ·········································   0.5ppb

脱甲基金霉素 ································   1.5ppb

土霉素 ···········································  1ppb

强力霉素 ········································  1.2ppb

u  交叉反应率

四环素 ··········································  100%

二甲胺四环素 ·································  125%

吡四环素  ·······································  110%

金霉素  ···········································  100%

脱甲基金霉素 ··································· 35%

土霉素  ············································  58%

强力霉素  ·········································  45%

u  样本回收率

鸡肉、猪肉、蜂蜜 ····························  85±20%

三、试剂盒组成

1微量测试孔每条8孔,一板12
2

标准品浓缩液

81ppb

1ml黑色帽
3

高浓度标准品

100ppb

1ml黑色帽

4

酶标记物

7ml

白色帽

5

抗体工作液

7ml

白色帽

6

底物A

7ml

白色帽

7

底物B

7ml

黑色帽

8

终止液

7ml

黄色帽

9

20X浓缩洗涤液

15ml

白色帽

10

10X样本提取液

50ml*2

透明帽

11

标准品复溶液

15ml*2

蓝色帽

四、样品前处理步骤及检测分析步骤

    详见产品说明书。

五、储藏条件和保质期

1、 储藏条件:试剂盒于2-8保存,不要冷冻。

2、 期:该产品有效期为1年,生产日期见包装盒。