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喹诺酮类(QNS)ELISA快速检测试剂盒(检组织/血清/蜂蜜,灵敏度1ppb,一步法)

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   喹诺酮类药物(QNS)ELISA快速检测试剂盒


一、原理

    试剂盒采用间接竞争ELISA方法,在微孔条上预包被偶联抗原,样本中的残留物喹诺酮类药物和微孔条上预包被的偶联抗原竞争抗喹诺酮类药物抗体,加入酶标记物后,用TMB底物显色,样本吸光值与其所含残留喹诺酮类药物的含量成负相关,与标准曲线比较即可得出相应残留物喹诺酮类药物的含量。


二、试剂盒特性

u  试剂盒灵敏度:          1ppb

u  孵育温度    :          25

u  孵育时间    :          30min15min

u  批内批间差     :     <15%

u  样本检测下限

恩诺沙星(ENR)检测下限  ···················· 1ppb

环丙沙星(CIF 检测下限  ··················· 1ppb

氧氟沙星(OFL 检测下限  ···················  1ppb

达氟沙星(DAN)检测下限  ···················· 1ppb

诺氟沙星(NOR)检测下限  ···················· 0.5 ppb

洛美沙星(LOM)检测下限  ···················· 1ppb

培氟沙星 PEF)检测下限  ····················0.5ppb

依诺沙星(ENO)检测下限  ···················· 1ppb

奥索利酸(OXO)检测下限  ···················· 1ppb

氟喹酸 (FLU)检测下限  ···················· 1ppb

麻保沙星(MAR)检测下限  ···················· 1ppb

氨氟沙星(AMI)检测下限  ···················· 1ppb

那氟沙星(NAD)检测下限  ····················  2ppb

u  交叉反应率

恩诺沙星(ENR   ···························  100%

环丙沙星(CIF   ···························  92.88%

氧氟沙星(OFL    ··························  98.86%

奥索利酸(OXO  ···························  116.86%

达氟沙星(DAN  ···························  102.63%

诺氟沙星(NOR  ···························  148.65%

洛美沙星(LOM  ···························   86.24%

培氟沙星(PEF   ···························  156.60%

依诺沙星(ENO  ···························   98.97%

氟喹酸(FLU    ···························   96.73%

麻保沙星(MAR  ···························  110.63%

氨氟沙星(AMI  ···························   98.86%

那氟沙星(NAD  ···························   56.81%

u  样本回收率

     ······································  80±15%

     ······································  80±15%

     ······································  75±15%


三、试剂盒组成

1

微量测试孔

每条8孔,一板12

2

标准液×6

1ml/瓶)

0ppb

1ppb

3ppb

9ppb

27ppb

81ppb

3

酶标记物

7ml

红色帽

4

抗体工作液

7ml

蓝色帽

5

底物A

7ml

白色帽

6

底物B

7ml

黑色帽

7

终止液

7ml

黄色帽

8

20X浓缩洗涤液

40ml

白色帽

9

2X浓缩复溶液

50ml

透明帽

四,样品前处理步骤及检测分析步骤

  详见产品说明书。

储藏条件和保质期

1、      储藏条件:试剂盒于2-8保存,不要冷冻。

2、      期:该产品有效期为1年,生产日期见包装盒。