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己烯雌酚快速检测试剂盒(检组织/饲料/尿样,灵敏度0.1ppb,两步法)

                  检测试剂盒12.jpg


 己烯雌酚(DES)快速检测试剂盒

一、原理

本试剂盒采用间接竞争ELISA方法,在微孔条上预包被偶联抗原,样本中的残留物己烯雌酚将和微孔条上预包被的偶联抗原竞争抗己烯雌酚抗体,加入酶标记物后,用TMB底物显色,样本吸光值与其所含残留物己烯雌酚的含量成负相关,与标准曲线比较即可得出相应残留物己烯雌酚的含量。

二、试剂盒特性

u  试剂盒灵敏度:      0.1 ppb

u  孵 育 温 度 :       37

u  孵 育 时 间 :       30min30min15min

u  板内、板间差:      小于10 %

u  样本检测下限

组织(虾、鱼) ·······························  0.2 ppb

猪肉/ 鸡肉/  ·······························  2  ppb

肌肉组织(方法二)  ···························  0.1 ppb

     ············································  20 ppb

尿      ···········································  0.6 ppb

u  交叉反应率

己烯雌酚  ········································  100%

双烯雌酚  ········································  38.5%

己烷雌酚  ········································  8.5%

己炔雌二醇  ···································  0.1%

雌三醇  ·········································  0.1%

u  样本回收率

  ··········································  90±10%

  ··········································  85±10%

尿  ··········································  70±10%

三、试剂盒组成

1

微量测试孔

每条8孔,一板12

2

标准液×6

1ml/瓶)

0ppb

0.1ppb

0.3ppb

0.9ppb

2.7ppb

8.1ppb

3

酶标记物

12ml

红色帽

4

抗体工作液

7ml

蓝色帽

5

底物A

7ml

白色帽

6

底物B

7ml

黑色帽

7

终止液

7ml

黄色帽

8

20X浓缩洗涤液

40ml

白色帽

9

5X浓缩复溶液

50ml

透明帽

四、样品前处理步骤及检测分析步骤

  详见产品说明书。

五、储藏条件和保质期

1、 储藏条件:试剂盒于2-8保存,不要冷冻。

2、 期:该产品有效期为1年,生产日期见包装盒。