Chinese  
   
磺胺类(SAS)多残留ELISA快速检测试剂盒(检组织/蜂蜜/血清/尿样/乳制品,灵敏度1ppb,一步法)

                           检测试剂盒12.jpg


磺胺类(SAS)多残留ELISA快速快速检测试剂盒

一、原理

本试剂盒采用间接竞争ELISA方法,在微孔条上预包被偶联抗原,样本中的残留物磺胺类药物将和微孔条上预包被的偶联抗原竞争抗磺胺类药物抗体,加入酶标记物后,用TMB底物显色,样本吸光值与其所含残留物磺胺类药物的含量成负相关,与标准曲线比较即可得出相应残留物磺胺类药物的含量。

二、试剂盒特性

u  试剂盒灵敏度:       1ppb

u  孵育温度    :       25

u  孵育时间    :       30min~15min

u 批内、批间差        不超过10%

u  样本检测下限

织( 法)  ·············   1ppb

织( 法)  ··············   5 ppb

················································   1ppb

尿   ···········································   4 ppb

      ··············································   20 ppb

u  交叉反应率

磺胺甲基嘧啶(SM1           ···························· 100%

磺胺嘧啶(SDSDZ           ····························  130.2%

磺胺间(六)甲氧嘧啶(SMM·························  181.8%

磺胺对甲氧嘧啶(SMD        ···························  194.8%

磺胺二甲基嘧啶(SM2  ···························  89.3%

磺胺二甲异嘧啶(SM2 ··························· 70.6%

磺胺间二甲氧嘧啶(SDM  ··························  140.8%

磺胺邻二甲氧嘧啶(SDM ··························  132.1%

磺胺甲噁唑(SMZ  ······························  86.6%

磺胺多辛(SDM2)   ······························  144.7%

酞酰磺噻唑(PST ······························  73.9%

磺胺甲噻二唑(SMT ·····························  69.4%

磺胺异噁唑(SIZ  ·····························  76.5%

磺胺噻唑(ST  ·······························  103.3%

磺胺喹噁林(SQX)  ······························    59.4%

磺胺甲氧哒嗪(SMP) ······························  108.6%

磺胺氯吡嗪(Esb3)  ······························  72.1%

磺胺氯哒嗪(SCPA)  ······························  59.6%

磺胺苯酰(SML)  ································  104%

由反应交叉率可以得出:该试剂盒可用于磺胺多种残留物的检测,完全满足国家磺胺类多残留种类检测的要求。

u  样本回收率

  、尿 样、牛   ···············   85% ±25%

蜜、血       ···························   80% ±23%

三、试剂盒组成

1

微量测试孔

每条8孔,一板12

2

标准液×6

1ml/瓶)

0ppb

1ppb

3ppb

9ppb

27ppb

81ppb

3

酶标记物

12ml

红色帽

4

抗体工作液

10ml

蓝色帽

5

底物A

7ml

白色帽

6

底物B

7ml

黑色帽

7

终止液

7ml

黄色帽

8

20X浓缩洗涤液

40ml

白色帽

9

20X浓缩复溶液

50ml

白色帽

四、样品前处理及检测分析步骤

     详见产品说明书。

五、储藏条件和保质期

1、 储藏条件:试剂盒于2-8保存,不要冷冻。

2、   期:该产品有效期为1年,生产日期见包装盒。