Chloramphenicol(CAP) rapid test dipsticks (Egg)

Catalog No. LSY-20004

1. Brief

This product is used for testing Chloramphenicol in egg, duck egg sample qualitatively. The whole test need 10min. Easy to operate, high sensitivity.

2. Detection limit

Honey:  0.3ng/ml (0.3ppb)

3. Contents

4. Principle

The Chloramphenicol rapid test dipstick is based on competitive inhibition immuno-chromatographic principle. In the flow process, Chloramphenicol in the sample combined with Chloramphenicol specific colloidal gold-labeled monoclonal antibody, Inhibit the combination between antibody and Chloramphenicol-BSA conjugate on Test line of NC membrane, lead to the color change of Test line. When the sample has no Chloramphenicol residue or concentration lower than detection limit, T line is darker than C line or T line has same color with C line; when the concentration is equal to or higher than detection limit, T line is obviously lighter than C line or T line has no color. No matter whether there is Chloramphenicol residue in sample, C line will appear, it means the test is valid.

5. Sample preparation

Eggs:take 1g fresh mixed well egg sample, add 2ml pure water, shake by hand or oscillator strongly for 3min, ready to test.

6.Operation procedures

6.1 Read the instruction carefully before use. Return test dipsticks and sample into room temperature.

6.2 Take bottles needed from the kit package, take out required microwells and dipsticks, making proper marks. Please use these test dipsticks within 1h. Seal the cap of the bottles, avoid moisture.

6.3 Take 200ul of the test samples into the microwells, then repeatedly absorb for 5 times, mix the sample with the reagent in the microwells completely, until no solid observed by eyes (this is a very important step).

6.4 Incubate for 5min at room temperature (20-25℃), insert the test dipsticks into the microwells with the "MAX" end fully dipped in to the mixture solution.

6.5 Insert the test dipsticks in microwells for 5-8min, read the result, it is invalid in other time.

7. Test Result Interpretation

7.1 Negative: T line is darker than C line, or T line has same color with C line. It means there is no Chloramphenicol residue in sample or the residue is lower than detection limit.

7.2 Positive: T line is obviously lighter than C line or T line is invisible. It means the Chloramphenicol residue is equal to or higher than detection limit.

7.3 Invalidation: C line isn’t seen wine red. It means the test strip is out of efficacy, out of date or improper operation. Please run the test again using another package. If the invalid tests keep happening, please contact the supplier.    

8.Precautions

1) The test strips can be used only once at room temperature, do not use test strips out of expiry date.

2) Please seal the bottle after taking out required test strips. If can not use all 8 micro-wells at once, cup the rest and put it back to bottle and sealed.

3) Do not touch the white membrane surface in the middle of test strips, avoid sunlight and fan blowing directly.

4) Use the test strips testing again for positive results.

5) Please contact the supplier for any questions.

6) The result is for reference only, for confirmation, please refer to the relevant national standard method.

9.Storage and expiry date

Storage:  Store at 2-8 ℃ in dark, sealed, dry place, no frozen.

Expiry date: 12 months; date of production is on box.