Tetracyclinesrapid test dipstick (Egg)

Catalog No. LSY-20046

1. Brief

This product is used for testing Tetracyclines residue in egg sample qualitatively. The total test time need 5-10 min. Easy to operate, high sensitivity.

2. Detection limit: 50ng/ml (50ppb)

3. Specifications: 8 dipsticks/bottle, 12 bottles/box

4. Principle

The Tetracyclines rapid test dipstick is based on competitive inhibition immuno-chromatographic principle. In the flow process, Tetracyclines in the sample combined with Tetracyclines specific colloidal gold-labeled monoclonal antibody, Inhibit the combination between antibody and Tetracyclines -BSA conjugate on Test line of NC membrane, lead to the color change of Test line.

When the sample has no Tetracyclines residue or concentration lower than detection limit, T line appear; when the concentration is equal to or higher than detection limit, T line has no color. No matter whether there is Tetracyclines residue in sample, C line will appear, it means the test is valid.  

5.Contents

6. Sample preparation 

Weigh 1g fresh mixed evenly egg sample, add 3ml pure water, shake by hand or oscillator strongly for 3min, ready to test.

7.Operation procedures

7.1 Read the instruction carefully before use. Return test dipsticks and sample into room temperature.

7.2 Take bottles needed from the kit package, take out required micro-wells and dipsticks, making proper marks. Please use these test dipsticks within 1h. Seal the cap of the bottles, avoid moisture. 

7.3 Take 200ul of the test samples into the micro-wells, then repeatedly pump and suck for many times, mix the sample with the reagent in the micro-wells completely until no solid judge by eyes (this is a very important step).

7.4 Incubate for 5min at room temperature (20-25℃), then insert the test dipsticks into the micro-wells with the "MAX" end fully dipped in to the mixture solution.

7.5 Insert the dipstick into the microwell for 5-8min, read the result, it is invalid in other time.

8. Test Result Interpretation

8.1 Negative: Both T line and C line appear. It means there is no Tetracyclines residue in sample or the residue is lower than detection limit.

8.2 Positive: C line appears, T line is invisible. It means the Tetracyclines residue is equal to or higher than detection limit.

8.3 Invalidation: C line isn’t seen wine red. It means the test card is out of efficacy, out of date or improper operation. Please run the test again using another package. If the invalid tests keep happening, please contact the supplier.

9.Specificity

This product has no cross reaction with Chloramphenicol, Quinolones, Streptomycin,Sulfonamide etc.

10.Precautions

1) The test strips can be used only once at room temperature, do not use strips out of expiry date.

2) Please seal the bottle after taking out required test dipsticks. If can not use all 8 micro-wells at once, cup the rest and put it back to bottle and sealed.

3) Do not touch the white membrane surface in the middle of test strip, avoid sunlight and fan blowing directly.

4) Eggs need to be fresh and non-polluting, and chilled eggs should be tested after returning to room temperature.

5) Test again for positive results.

6) Please contact the supplier for any questions.

7) The test results are for reference only. For confirmation, please refer to the relevant national standard methods.

11.Storage and expiry date

Storage:  Store at 2-8 ℃ in dark, sealed, dry place, no frozen.

Expiry date: 12 months; date of production is on box.