Quinolones rapid test strips (Egg)
Catalog No. LSY-20047
1. Specifications:8 dipsticks/bottle, 12 bottles/box
2. Brief
This product is used for testing Quinolones in egg, duck egg sample qualitatively. The whole test time only need 10min, easy to operate with high sensitivity.
3. Detection limit
Name
|
Detection limit
|
Name
|
Detection limit
|
Enrofloxacin
|
3-5ppb
|
Difloxacin
|
3-5ppb
|
Ciprofloxacin
|
3-5ppb
|
Oxolinic acid
|
3-5ppb
|
Norfloxacin
|
3-5ppb
|
Enoxacin
|
5-6ppb
|
Pefloxacin
|
3-5ppb
|
Marbofloxacin
|
5-6ppb
|
Sarafloxacin
|
5-6ppb
|
Flumequine
|
3-5ppb
|
Danofloxain
|
5-6ppb
|
Lomefloxacin
|
5-6ppb
|
Ofloxacin
|
3-5ppb
|
|
|
4. Contents
1
|
Quinolones test strip
|
8 strip/bottle, 12 bottles
|
2
|
Manual
|
1piece
|
3
|
Desiccant
|
2pieces/bottle
|
5. Principle
The Quinolones rapid test strip is based on competitive inhibition immuno-chromatographic principle. In the flow process, Quinolones in the sample combined with Quinolones specific colloidal gold-labeled monoclonal antibody, Inhibit the combination between antibody and Quinolones-BSA conjugate on Test line of NC membrane, lead to the color change of Test line. When the sample has no Quinolones residue or concentration lower than detection limit, T line is darker than C line or T line has same color with C line; when the concentration is equal to or higher than detection limit, T line is obviously lighter than C line or T line has no color. No matter whether there is Quinolones residue in sample, C line will appear, it means the test is valid.
6. Sample preparation
Weigh 1g fresh mixed evenly egg sample, add 3ml pure water, shake by hand or oscillator strongly for 3min, ready to test.
7.Operation procedures
7.1 Read the instruction carefully before use. Return test strips and sample into room temperature.
7.2 Take bottles needed from the kit package, take out required microwells and strips, making proper marks. Please use these test strips within 1h. Seal the cap of the bottles, avoid moisture.
7.3 Take 200ul of the test samples into the micro-wells, then repeatedly pump and suck for 5 times, mix the sample with the reagent in the micro-wells completely until no solid judge by eyes(this is a very important step).
7.4 Incubate for 5min at room temperature (20-25℃), then insert the test strips into the micro-wells with the "MAX" end fully dipped in to the mixture solution.
7.5 Insert the test strips into micro-wells for 5-8min then read the result, it is invalid in other time.
8. Test Result Interpretation
8.1 Negative: T line is darker than C line, or T line has same color with C line. It means there is no Quinolone residue in sample or the residue is lower than detection limit.
8.2 Positive: T line is obviously lighter than C line or T line is invisible. It means the Quinolone residue is equal to or higher than detection limit.
8.3 Invalidation: C line isn’t seen wine red. It means the test card is out of efficacy, out of date or improper operation. Please run the test again using another package. If the invalid tests keep happening, please contact the supplier.
9.Precautions
1) The test strips can be used only once at room temperature, do not use strips out of expiry date.
2) Please seal the bottle after taking out required test dipsticks. If can not use all 8 micro-wells at once, cup the rest and put it back to bottle and sealed.
3) Do not touch the white membrane surface in the middle of test strip, avoid sunlight and fan blowing directly.
4) Eggs need to be fresh and non-polluting, and chilled eggs should be tested after returning to room temperature.
5) Test again for positive results.
6) Please contact the supplier for any questions.
7) The test results are for reference only. For confirmation, please refer to the relevant national standard methods.
10. Specificity
Test 500ppb Sulfonamides, Chloramphenicol and tetracyclines and other drugs. All the results are negative.
11.Storage and expiry date
Storage: Store at 2-8 ℃ in dark, sealed, dry place, no frozen.
Expiry date: 12 months; date of production is on box.