Rotavirus antigen rapid test kit (Colloidal gold method)
Catalog No. LSY-20091
1.
Introduction
The Rotavirus antigen rapid test kit is
based on colloidal gold immuno-chromatography technique to detect type A
Rotavirus antigen in feces of cattle, pig, dog and cat. After adding
sample, the sample move along with the colloidal gold labeling Rotavirus antibody, if there is Rotavirus antigen in the sample, it combines with antibody on T
line and show wine-red color; if there is no Rotavirus antigen, then no color reaction.
2. Contents
|
1
|
RV Ag test
card
|
40 pieces
|
|
2
|
Swab
|
40 pieces
|
|
3
|
Dropper
|
40 pieces
|
|
4
|
Sample
buffer
|
40 bottles
|
|
5
|
Manual
|
1 piece
|
3. Test procedure
1) Use swab to collect fresh
feces or take sample from Rectum directly. If it is for Rotavirus screening for
animals without diarrhea, please take sample from Rectum. We suggest that do
not take dry feces sample, because collecting dry feces sample will lead to
collecting too much sample and appear false positive.
2) Insert the swab into the
tube with sample buffer immediately, rotate the swab strongly on tube well for
at least 10 times and mix the solution evenly, making the sample been dissolved
in sample buffer fully. Press the swab on tube well above the liquid level,
making the liquid been pressed out fully, discard swab.
3) If the feces particles is
big, be static for 1-2min, make big particles precipitate.
4) Take out test card from
sealed bag. Put it on flat surface, use dropper to draw upper-layer bright
liquid, drop 5 drops (do not over adding) slowly, one by one, to the sample hole with “S”
mark.
5) Put the test card at room
temperature for 10-15min, read the result, the result is invalid after 15
minutes.
4. Results
judgment
1) Positive: control line and test line are both seen
wine red;
2) Negative: only control line is seen wine red;
3) Invalidation: control line isn’t seen wine red.
5. Note
1). Products that have expired or have damaged aluminum foil packaging
bags cannot be used.
2). The test card must be used within 1 hour after removal.
3). Please use the accompanying sample buffer. Do not mix Sample buffer
between different products or Sample buffer from different batches of the same
product.
4). The collected sample should be tested immediately, avoid long time
storage.
5). The
collection of samples directly affects the accuracy of testing, so ensuring the
correct, sufficient, and appropriate collection of samples. Excessive
collection of fecal samples can affect the normal flow of fluids and lead to
incorrect results (black bands appearing), while too few samples may result in
false negative results. Therefore, please control the sampling amount according
to the diagram
6). Avoiding bubbles when adding samples to the sample hole of the test
card. When adding samples, please keep the front end of the straw about 1 cm
away from the sample hole to ensure accurate adding samples; If the distance is
too close, the drop volume may be reduced or the sample size may not be added
due to the error in judging the number of drops, which will affect the test
effect
7). Used test cards and samples should be considered as pollutants and
must be properly disposed of in accordance with relevant local regulations
6. Frequently Asked Questions
1) Limitation of the product
This kit is a qualitative screening reagent, can detect RV antigen
accurately, but if sample is not enough or concentration of antigen is lower
than sensitivity, it may appear negative result. A clear clinical diagnosis
should be made after taking into account all clinical and laboratory
phenomenon.
2) The sensitivity and specificity of the product.
Compare with PCR, the sensitivity > 95%, it has no cross-reaction with
similar symptoms to those of Coronavirus, adenovirus, Canine parvovirus,
Escherichia coli, infectious gastroenteritis, epidemic diarrhea virus, bovine
infectious diarrhea virus
3) Whether there will be Positive reaction after vaccination.
RV vaccination won’t make RV antigen appear in feces samples, so
vaccination won’t make positive result.
7. Storage and expiry date
Specifications: 40T/kit
Storage: Store at 2-30 ℃ (No frozen). Return to
room temperature before open the Aluminum foil bags for cold storage.
Expiry date: 24 months; date of
production is on box.
Shenzhen Lvshiyuan
Biotechnology Co., Ltd
D
Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road,
Dapeng, Shenzhen, 518120 China
Tel.
86-755-28438788
Fax
86-755-28938800
Email: info@lsybt.com
www.lsybt.com