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How to add sample for Food Safety ELISA kit

浏览次数: 3827 发布时间: 2019-11-15 11:57

How to add sample for Food Safety ELISA kit


1. Add Standard and Sample into micro-wells as shown in the following sheet, 50ul/well:

1-1  Single-well test

 

1

2

3

4

5

6

7

8

9

10

11

12

A

Standard 1

Sample 3

 

 

 

 

 

 

 

 

 

 

B

Standard 2

Sample 4

 

 

 

 

 

 

 

 

 

 

C

Standard 3

Sample 5

 

 

 

 

 

 

 

 

 

 

D

Standard 4

Sample 6

 

 

 

 

 

 

 

 

 

 

E

Standard 5

Sample 7

 

 

 

 

 

 

 

 

 

 

F

Standard 6

Sample 8

 

 

 

 

 

 

 

 

 

 

G

Sample 1

Sample 9

 

 

 

 

 

 

 

 

 

 

H

Sample 2

Sample 10

 

 

 

 

 

 

 

 

 

 

1-2  Double-well test

 

1

2

3

4

5

6

7

8

9

10

11

12

A

Standard 1

Standard 5

Sample 3

Sample 7

 

 

 

 

 

 

 

 

B

Standard 1

Standard 5

Sample 3

Sample 7

 

 

 

 

 

 

 

 

C

Standard 2

Standard 6

Sample 4

Sample 8

 

 

 

 

 

 

 

 

D

Standard 2

Standard 6

Sample 4

Sample 8

 

 

 

 

 

 

 

 

E

Standard 3

Sample 1

Sample 5

Sample 9

 

 

 

 

 

 

 

 

F

Standard 3

Sample 1

Sample 5

Sample 9

 

 

 

 

 

 

 

 

G

Standard 4

Sample 2

Sample 6

Sample 10

 

 

 

 

 

 

 

 

H

Standard 4

Sample 2

Sample 6

Sample 10

 

 

 

 

 

 

 

 

 

2. Add Enzyme Conjugate, 50ul/well into micro-wells already add standard and sample

3. Add Antibody working solution, 50ul/well into micro-wells already add standard/sample and Enzyme conjugate, cover and shake gently, incubate at 25℃ for 30min.

 

 

Shenzhen Lvshiyuan Biotechnology Co., Ltd  


 

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Company: Shenzhen Lvshiyuan Biotechnology Co.,Ltd

WeChat/WhatsApp: +86-13427908554

Mobile: +86-18165709090 Skype: bellazou3

E-mail: info@lsybt.com, lsy@lsybt.com

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