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LSY-20044 Beta-lactam antibiotics rapid test strip

β-lactam antibiotics rapid test strip  

Catalogue No.: LSY-20044

1. Brief

The β-lactam antibiotics rapid test strip, based on Colloidal gold immunochromatography assay method, is used for testing β-lactam antibiotics residue in milk and goat milk.

2. Specifications

(1) 8 microwells and 8 dipsticks in one bottle      12 bottles / kit.

(2) Instruction                                        1 piece

3. Principle

The β-lactam antibiotics rapid test dipstick is based on competitive inhibition immuno-chromatographic principle. When the β-lactam antibiotics residue in sample is higher than or equal to the detection limit, it will combine with labled antibody, then inhibit the combination between colloidal gold antibody coated antigen on T line, making T line lighter than C line or T line invisible or T line has same color with C line. Otherwise, T line is darker than C line. No matter whether there is β-lactam antibiotics residue in sample, C line is visible.

 4. Detection limit

β-lactam Name

Detection

Limit (ppb)

β-lactam Name

Detection

Limit (ppb)

Penicillin G

2-4

Cefquinome

8-15

Amoxicillin

2-3

Cefalotin

30-40

Ampicillin

2-3

Cefazolin

40-50

Oxacillin

5-7

Cephalonium

8-10

Cloxacillin

4-6

Cefacetrile

20-30

Piperacillin

6-8

Cefoperazone

3-5

Nafcillin

15-25

Cephapirin

8-10

Dicloxacillin

6-8

Ceftiofur

60-100

5. Operation procedures

1) Read the manual carefully before testing, return test dipsticks and milk sample into room temperature before use.

2) Absorb 200ul milk sample into Micro-well, sucking 5~10 times until sample mix even with reagent in micro-well, observe by eyes no solid.

3) Incubate at 40℃ for first step[Recommended, if no incubator, incubate at room temperature(25℃) is also OK], timing for 3min;

4) Insert the dipstick into micro-well, put the end with MAX down, incubate at 40℃ for second step[Recommended, if no incubator, incubate at room temperature(25℃) is also OK], timing for 3min

5) Take out the dipstick from micro-well, judge the result.

6. Test Result Interpretation

Color depth comparison of  T line and C line

Result judgment

Result analysis

T line > C line

Negative

β-lactam antibiotics residue is lower than detection limit in sample

T line = C line

Weak positive

β-lactam antibiotics residue is equal to detection limit in sample

T line < C line or T line is invisible

Positive

β-lactam antibiotics residue is higher than detection limit in sample

C line is invisible

Invalid

The test dipstick is invalid, test again

7. Results picture show

Beta-lactam.jpg 


8. Precautions

1) This product is suitable for pure milk of cattle and sheep and raw milk.

2) Test dipsticks and micro-wells are disposable,do not use test dipsticks out of date.

3) Please seal the bottle after taking out required test dipsticks. If can not use all 8 microwells at once, cup the rest and put it back to bottle and sealed.

4) Do not touch the white membrane surface in the middle of test dipsticks, avoid sunlight and fan blowing directly.

5) Store milk sample in cold, no more than 3 days.

6) Milk samples should be fully liquid without any agglomeration, sour and precipitation. Stir milk sample to even before test.

7) For milk sample with high fat content, chromatography is slow, recommending extension the second step for another 1 min.

8) Use the test dipsticks testing again for positive results.

9. Specificity

This product has no cross-reaction with Tetracyclines, Quinolones, Sulfonamides, Chloramphenicol and Melamine etc.

 

10. Storage and expiry date

Storage:  Store at 2-8 ℃ in sealed, dry, and dark place, no frozen. 

Expiry date: 12 months; date of production is on box.