Neomycin rapid test strip (Egg)
Catalog No.: LSY-20041G5
1. Brief
This product is used for testing Neomycin residue in egg, duck eggs
etc. sample qualitatively. The total test time only needs 10 min. Easy to
operate, high sensitivity.
2. Detection limit: 50μg/kg(ppb)
3. Specifications: 96tests/box
4. Principle
The Neomycin rapid test strip is based on competitive inhibition immuno-chromatographic principle. In the flow process, Neomycin in the sample combined with Neomycin specific
colloidal gold-labeled monoclonal antibody, Inhibit the combination between
antibody and Neomycin-BSA conjugate on Test line of NC membrane, lead to
the color change of Test line.
5. Contents
|
1
|
Neomycin rapid test strip and micro-well
reagent
|
16 pieces/bottle, 6
bottles
|
|
3
|
Manual
|
1 piece
|
|
5
|
10X Sample extraction
buffer
|
1 bottle(50ml/bottle)
|
6. Materials needed but not
provided.
Instruments: electronic balance, micropipette, vortex
oscillator, homogenizer.
Consumables: 15/50ml centrifuge tube, disposable tips.
7. Sample preparation
Dilute 10X Sample extraction buffer with deionized water at 10 times before use. (1
part of 10X Sample extraction A + 9 parts of deionized water)
Sample
preparation
Take 1g of fresh and evenly mixed egg sample, add 4ml of sample
extraction buffer, vigorously shake by hand or vortex shaker for 1 minute, ready
for testing.
8. Operation
procedures
8.1 Read the instruction
carefully before use. Return test strips and sample into room temperature.
8.2 Take bottles needed
from the kit package, take out required micro-wells and dipsticks, making
proper marks. Please use these test dipsticks within 1h. Seal the cap of the
bottles, avoid moisture.
8.3 Take 200µl of the test samples
into the micro-wells, then repeatedly pump and suck for many times, mix the
sample with the reagent in the micro-wells completely until no solid judge by
eyes (this is a very important step).
8.4 Incubate for 5min at room temperature (20-40℃), then insert the test strips into the micro-wells with the
"MAX" end fully dipped in to the mixture solution.
8.5 After immersing the strips
into the microwell for 5min, take out the strip, then read the result
within 1min, it is invalid in other time.
9. Test Result Interpretation
9.1 Negative: C line
appears, T line is darker than C line, or T line has same color with C line. It
means there is no Neomycin residue in sample or the residue is lower than detection limit.
9.2 Positive: C line appears, T line is obviously lighter
than C line or T line is invisible. It means the Neomycin residue is equal to or higher than detection limit.
9.3 Invalidation: C line isn’t seen wine
red. It means the test card
is out of efficacy, out of date or improper operation. Please run the test
again using another package. If the invalid tests keep happening, please
contact the supplier.
10. Precautions
1). The test strip is disposable at room
temperature; Do not use expired test strips.
2). Disposable tips cannot be reused to avoid
cross contamination.
3). Try not to touch the white film surface in
the center of the test strip during use; Avoid direct sunlight and direct fan
blowing.
4). The egg sample must be a uniform liquid,
without clumping, souring, or precipitation.
5). Tap water, distilled water, deionized water,
or sample extracts cannot be used as negative controls.
6). If a positive result appears, it is
recommended to retest with this test strip.
7). Due to differences in samples, some
detection lines may appear lighter or grayish in color, but as long as a red
band appears, it can be determined.
8). If you encounter any problems during the
experiment, please contact our company.
11. Safety performance and garbage disposal
This product's test strips and packaging are
non-toxic and harmless, and should be disposed of as dry waste after use.
12. Storage
and expiry date
Storage: Store at 2-8 ℃ in dark,
sealed, dry place, no frozen.
Expiry
date: 12 months; date of production is on box.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building,
National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng,
Shenzhen, 518120 China
Tel.
86-755-28438788 Fax 86-755-28938800
Email: info@lsybt.com
Website: www.lsybt.com