Gentamicin rapid test strip (Honey)
Catalog No.: LSY-20043H2
1. Brief
This product is used for testing Gentamicin residue in honey sample qualitatively.
The total test time only needs 15-30 min. Easy to operate, high sensitivity.
2. Detection limit: 1μg/kg(ppb)
3. Specifications: 96tests/box
4. Principle
The Gentamicin rapid test strip is based on competitive inhibition immuno-chromatographic principle. In the flow process, Gentamicin in the sample combined with Gentamicin specific
colloidal gold-labeled monoclonal antibody, Inhibit the combination between
antibody and Gentamicin-BSA conjugate on Test line of NC membrane, lead to
the color change of Test line.
5. Contents
|
1
|
Gentamicin rapid test strip and micro-well
reagent
|
8 pieces/bottle, 12
bottles
|
|
3
|
Manual
|
1 piece
|
|
5
|
10X Sample extraction
buffer
|
1 bottle
|
6. Materials needed but not
provided.
Instruments: electronic balance, centrifuge, micropipette,
vortex oscillator, homogenizer.
Consumables: 50ml centrifuge tube, disposable tips.
7. Sample preparation
Dilute 10X Sample extraction buffer with deionized water at 10 times before use. (1
part of 10X Sample extraction A + 9 parts of deionized water)
Sample
preparation
Weigh 1±0.05g honey, add 3ml diluted Sample extraction
buffer, shake for 3min to dissolve it evenly, ready to test.
8. Operation
procedures
8.1 Read the instruction
carefully before use. Return test strips and sample into room temperature.
8.2 Take bottles needed
from the kit package, take out required micro-wells and dipsticks, making
proper marks. Please use these test dipsticks within 1h. Seal the cap of the
bottles, avoid moisture.
8.3 Take 200µl of the test samples
into the micro-wells, then repeatedly pump and suck for many times, mix the
sample with the reagent in the micro-wells completely until no solid judge by
eyes (this is a very important step).
8.4 Incubate for 5min at room temperature (20-40℃), then insert the test strips into the micro-wells with the
"MAX" end fully dipped in to the mixture solution.
8.5 After immersing the strips
into the microwell for 8-10min,take out the strip, then read the result
within 1min, it is invalid in other time.
9. Test Result Interpretation
9.1 Negative: C line
appears, T line is darker than C line, or T line has same color with C line. It
means there is no Gentamicin residue in sample or the residue is lower than detection limit.
9.2 Positive: C line appears, T line is obviously lighter
than C line or T line is invisible. It means the Gentamicin residue is equal to or higher than detection limit.
9.3 Invalidation: C line isn’t seen wine
red. It means the test card
is out of efficacy, out of date or improper operation. Please run the test
again using another package. If the invalid tests keep happening, please
contact the supplier.
10. Precautions
1). The test strip is disposable at room
temperature; Do not use expired test strips.
2). Disposable tips cannot be reused to avoid
cross contamination.
3). Try not to touch the white film surface in
the center of the test strip during use; Avoid direct sunlight and direct fan
blowing.
4). Tap water, distilled water, or deionized
water cannot be used as negative controls.
5). If a positive result appears, it is
recommended to retest with this test strip.
6). If you encounter any problems during the
experiment, please contact our company.
11. Safety performance and garbage disposal
This product's test strips and packaging are
non-toxic and harmless, and should be disposed of as dry waste after use.
12. Storage
and expiry date
Storage: Store at 2-8 ℃ in dark,
sealed, dry place, no frozen.
Expiry
date: 12 months; date of production is on box.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building,
National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng,
Shenzhen, 518120 China
Tel.
86-755-28438788
Fax
86-755-28938800
Email: info@lsybt.com
Website: www.lsybt.com